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mCherry-Cre lentivirus vector

Catalog# Unit Unit Price (USD) Actions
CRE03 10 μg at 0.5 μg/μL in TE $695.00 Add to Cart
Description:

The Cre/LoxP recombination system is a versatile and powerful tool for DNA manipulations such as deletions, insertions, translocations, and inversions at specific sites in the DNA of cells.  The system uses Cre recombinase to recombine a pair of Lox sites.  Depending on the direction and distance of the LoxP sites, DNA flanked by the LoxP sites can be deleted, inversed, or translocated.

The mCherry-Cre Lentivirus Vector (pLenti-EF1-mCherry-T2A-Cre-PGK-PURO) expresses the mCherry and Cre and provides puromycin resistance to the cell receiving it. This lentivirus vector can be used for transient Cre expression and for stable cell line development. Robust Cre recombinase activity has been detected in stable transgenic cell lines that carry two loxP sites in the same direction in their transgenes. DNA deletion event was detected by PCR and validated by DNA sequencing.

Vector Information

This is a lentiviral expression vector containing all elements for efficient and high yield viral production. A ubiquitous EF1 drives the expression of mCherry and Cre while PGK drives Puro to allow for selection of transduced cells. This vector can be used for transient Cre expression and stable cell line generation.

IMPORTANT NOTICE

Store the vial at -20°C immediately upon receipt.

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Note: Bacterial culture of pLenti vectors should be done in medium containing 50 μg/mL Ampicillin. For maximal plasmid yield and quality, we recommend Stbl3 competent cells (Invitrogen).
Specifications:
Product Name mCherry-Cre lentivirus vector
Shipping Condition

Room Temperature - 2 Day Shipping

Storage and Stability

Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

This plasmid is sequence verified.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.