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Human iPS Cells (Inducible A-N, ApoE3)

Catalog# Unit Unit Price (USD) Actions
iP26DN 5x105 cells/vial $11,988.00 Add to Cart
Highlights:

ALSTEM offers human iPS cell line with inducible expression of ATOH1 and NGN2 by doxycycline (Dox). This cell line is generated from pre-made footprint-free human iPS cells with the inducible Ngn2 that has been stably integrated into the human AAVS1 “Safe Harbor” site. It provides a convenient means to differentiate human iPS cells to dopaminergic neurons rapidly and efficiently. The integration at “Safe Harbor” sites ensures safe and reliable expression of Ngn2 when Dox is supplied.

  • Highly responsive doxycycline inducible iPS cell line
  • Ideal for in vitro rapid dopaminergic neuronal differentiation
Description:

Overexpression of the transcription factors Atonal BHLH Transcription Factor 1 (ATOH1) and Neurogenin 2 (NGN2) promotes direct differentiation of pluripotent stem cells to functional dopaminergic neurons. AAVS1 (also known as PPP1R12C) locus supports robust and sustained transgene expression. Precise insertion of tetracycline inducible ATOH1 and NGN2 (A-N) into human iPS cells at AAVS1 site could generate a stable iPSC line for rapid dopaminergic neuron differentiation upon induction. The highly responsive doxycycline inducible ATOH1 and NGN2-expressing human iPS cell line cat# iP26DN) is developed from pre-made footprint-free human iPS cells (cat# iPS26, ApoE3 isoform) by integrating an all-in-one inducible NGN2 and ATOH1 cassette into the human AAVS1 “Safe Harbor” site. This cell line is ideal for rapid in vitro neuronal differentiation.

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The all-in-one cassette consisting of both inducible NGN2 and ATOH1 and constitutive M2rtTA has been inserted into one of the AAVS1 alleles by gene editing through HDR pathway, while the other allele remains intact. Upon induction, NGN2 and ATOH1 is expressed and the iPS cells will be directly differentiated into dopaminergic neurons.
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Upon doxycycline treatment, inducible A-N iPS cells differentiate to dopaminergic neurons. Six days after Dox treatment, majority of cells have converted to dopaminergic neurons (tyrosine hydroxylase (TH) positive) with extensive growth of axons and dendrites.
Specifications:
Product Name Human iPS Cells (Inducible A-N, ApoE3)
Shipping Condition

Dry Ice - Overnight Shipping

Storage and Stability

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed

Quality Control

Human iPS cells (inducible A-N, ApoE3) were grown in feeder-free conditions with mTeSR1 medium. Each lot of hiPSCs is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and OCT4.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.