pAAVS1-SA-T2A-Neo-CAG-MCS-SV40pA Donor Vector
| Catalog# | Unit | Unit Price (USD) | Actions |
|---|---|---|---|
| AC2302 | 10 μg at 0.5 μg/μL in TE | $950.00 | Add to Cart |
Description:
Human AAVS1 donor vectors are meticulously designed to target the human AAVS1 "safe harbor" site nestled within intron 1 of the PPP1R12C gene (protein phosphatase 1 regulatory subunit 12C). This specific locus is aptly called a "safe harbor" because it exerts minimal influence on cellular functions. It excels in supporting robust transcription, making it an ideal site for maintaining the expression of an externally introduced gene while mitigating the risk of unintentional integration elsewhere. Our latest-generation donor vectors incorporate a multiple cloning site (MCS) strategically positioned downstream of the CAG promoter and upstream of a poly-A tail. This arrangement ensures a streamlined and straightforward path to achieve enduring and strong expression of the Gene of Interest (GOI). Furthermore, leveraging the intronic location of the AAVS1 safe harbor locus, the Neomycin selection marker is thoughtfully integrated with a splice acceptor (SA) site, devoid of its own promoter. This design guarantees that Neomycin expression can only occur when the construct integrates within an intron. Consequently, Neomycin expression becomes intricately linked to the PPP1R12C transcript, significantly reducing the risk of unintended off-target integrations when employing Neomycin selection. The pAAVS1-SA-T2A-Neo-CAG-MCS-SV40pA Vector plays a pivotal role in the Alstem pAAVS1/Cas9 Knock-In vector system. It facilitates robust expression of a GOI through the utilization of the CAG promoter and incorporates the convenient Neomycin selection marker for ease of screening. When used in conjunction with the pspCAS9 nuclease and gRNA vector, it enables the seamless integration of the GOI into the AAVS1 safe harbor site, simplifying the process of enhancing GOI expression.
Vector Information
This is a AAVS1 donor vector that contains the necessary elements for introducing a GOI (gene of interest) to AAVS1 Safe Harbor site. A ubiquitous SA-T2A-NeoR cassette ensures the intergration of GOI in AAVS1 safe harbor sites by antibiotic selection. The CAG promoter drives the expression of the GOI. This vector can be used for stable cell line generation.
IMPORTANT NOTICE
Store the vial at -20°C immediately upon receipt.
Specifications:
| Product Name | pAAVS1-SA-T2A-Neo-CAG-MCS-SV40pA Donor Vector |
|---|---|
| Shipping Condition |
Room Temperature - 2 Day Shipping |
| Storage and Stability |
Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed. |
| Quality Control | This plasmid is sequence verified. |
| Restricted Use |
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |