Description:

Human AAVS1 donor vectors are meticulously designed to target the human AAVS1 "safe harbor" site nestled within intron 1 of the PPP1R12C gene (protein phosphatase 1 regulatory subunit 12C). This specific locus is aptly called a "safe harbor" because it exerts minimal influence on cellular functions. It excels in supporting robust transcription, making it an ideal site for maintaining the expression of an externally introduced gene while mitigating the risk of unintentional integration elsewhere. Our latest-generation donor vectors incorporate a multiple cloning site (MCS) strategically positioned downstream of the SFFV promoter and upstream of a poly-A tail in a reverse orientation. This arrangement ensures a streamlined and straightforward path to achieve more consistent and robust expression of the Gene of Interest (GOI). Furthermore, leveraging the intronic location of the AAVS1 safe harbor locus, the Neomycin selection marker is thoughtfully integrated with a splice acceptor (SA) site, devoid of its own promoter. This design guarantees that Neomycin expression can only occur when the construct integrates within an intron. Consequently, Neomycin expression becomes intricately linked to the PPP1R12C transcript, significantly reducing the risk of unintended off-target integrations when employing Neomycin selection. When the pAAVS1-SA-T2A-Neo-SV40pA-MCS-SFFV Donor Vector co-transfected with the pspCAS9 nuclease and gRNA vector, it creates the seamless integration of the GOI into the AAVS1 safe harbor site, simplifying the process of enhancing GOI expression.

Vector Information

This is an AAVS1 donor vector that contains the necessary elements for introducing a GOI (gene of interest) to the AAVS1 Safe Harbor site. A ubiquitous SA-T2A-NeoR cassette ensures the intergration of the GOI in the AAVS1 safe harbor site by antibiotic selection. The SFFV promoter drives the expression of the GOI. This vector can be used for stable cell line generation.

IMPORTANT NOTICE

Store the vial at -20°C immediately upon receipt.

Specifications:

Product Name	pAAVS1-SA-T2A-Neo-SV40pA-MCS-SFFV Donor Vector
Shipping Condition	Room Temperature - 2 Day Shipping
Storage and Stability	Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control	This plasmid is sequence verified.
Restricted Use	For Research Use Only. Not for use in diagnostic or therapeutic procedures.