pAAVS1-SA-T2A-Neo-CAG-dCas9-VPR-pA Donor Vector
| Catalog# | Unit | Unit Price (USD) | Actions |
|---|---|---|---|
| AC8302 | 10 μg at 0.5 μg/μL in TE | $1,150.00 | Add to Cart |
Description:
CRISPR activation (CRISPRa) is emerging as a potent tool, allowing targeted activation of gene transcription in both prokaryotic and eukaryotic cells. The synergy of the inactive Cas9 (dCas9) and the VP64-p65-Rta (VPR) activator enables accurate and reversible gene activation. Furthermore, the strategic utilization of the Human AAVS1 “safe harbor” site, located within intron 1 of the PPP1R12C gene, minimizes its impact on cellular functions. Our innovative pAAVS1-SA-T2A-Neo- CAG-dCas9-VPR-pA donor vector seamlessly combines the strengths of CRISPRa and the AAVS1 safe harbor site. Featuring a CAG promoter-driven CRISPR-VPR fusion gene, this design ensures consistent and robust expression of the dCas9/VPR protein without disrupting cellular functions. The vector also strategically integrates a Neomycin selection marker with a splice acceptor (SA) site within the AAVS1 safe harbor locus, closely tying Neomycin-resistant gene expression to intron integration. This significantly reduces the risk of unintended off-target integrations during G418 selection. When employed with the SpCas9 nuclease and AAVS1 gRNA expression vector, the pAAVS1-SA-T2A-Neo-CAG-dCas9-VPR-pA donor vector facilitates the seamless integration of a dCas9/VPR fusion protein into the AAVS1 safe harbor site. This approach, combined with specific gene-targeted gRNAs, enables precise and highly efficient activation of the target gene expression, offering an easy and consistent method for specific gene expression activation.
Vector Information
This AAVS1 donor vector includes essential components to serve as a reliable integration marker in the AAVS1 Safe Harbor site. It incorporates a universal SA-T2A-NeoR cassette, facilitating the integration of dCas9/VPR fusion gene driven by a CAG promoter into the AAVS1 safe harbor site through antibiotic selection. This vector is suitable for generating CRISPRa stable cell lines.
IMPORTANT NOTICE
Store the vial at -20°C immediately upon receipt.
Specifications:
| Product Name | pAAVS1-SA-T2A-Neo-CAG-dCas9-VPR-pA Donor Vector |
|---|---|
| Shipping Condition |
Room Temperature - 2 Day Shipping |
| Storage and Stability |
Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed. |
| Quality Control | This plasmid is sequence verified. |
| Restricted Use |
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |