Description:

Human AAVS1 donor vectors are meticulously designed to target the human AAVS1 "safe harbor" site nestled within intron 1 of the PPP1R12C gene (protein phosphatase 1 regulatory subunit 12C). This specific locus is aptly called a "safe harbor" because it exerts minimal influence on cellular functions. It excels in supporting robust transcription, making it an ideal site for maintaining the expression of an externally introduced gene while mitigating the risk of unintentional integration elsewhere. Our latest-generation control donor vectors incorporate a EGFP gene strategically positioned downstream of the CAG promoter and upstream of a poly-A tail. This setup ensures a straightforward pathway for achieving enduring and strong EGFP expression. Furthermore, by strategically integrating the Puromycin selection marker with a splice acceptor (SA) site within the intronic region of the AAVS1 safe harbor locus, independent of its own promoter, Puromycin expression becomes closely tied to intron integration. This significantly reduces the risk of unintended off-target integrations when employing Puromycin selection. The pAAVS1-SA-T2A-Puro-CAG-copGFP-SV40pA Vector, when used in conjunction with the pSpCAS9 nuclease & gRNA vector, enables the seamless integration of copGFP into the AAVS1 safe harbor site, making it an effective integration marker.

Vector Information

This AAVS1 donor vector includes essential components to serve as as a reliable integration marker in the AAVS1 Safe Harbor site. It incorporates a universal SA-T2A-Puro cassette, facilitating the integration of copGFP controlled by CAG promoter into the AAVS1 safe harbor site through antibiotic selection. This vector is suitable for generating stable cell lines.

IMPORTANT NOTICE

Store the vial at -20°C immediately upon receipt.

Specifications:

Product Name	pAAVS1-SA-T2A-Puro-CAG-copGFP-SV40pA Control Donor Vector
Shipping Condition	Room Temperature - 2 Day Shipping
Storage and Stability	Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control	This plasmid is sequence verified.
Restricted Use	For Research Use Only. Not for use in diagnostic or therapeutic procedures.