Banner

Products

All Products Gene Editing AAVS1 Insertion Vectors pAAVS1-SA-T2A-Puro-SV40pA-EGFP-SFFV Control Donor Vector

pAAVS1-SA-T2A-Puro-SV40pA-EGFP-SFFV Control Donor Vector

Catalog# Unit Unit Price (USD) Actions
AC9411 10 μg at 0.5 μg/μL in TE $950.00 Add to Cart
Description:

Human AAVS1 donor vectors are meticulously designed to target the human AAVS1 "safe harbor" site nestled within intron 1 of the PPP1R12C gene (protein phosphatase 1 regulatory subunit 12C). This specific locus is aptly called a "safe harbor" because it exerts minimal influence on cellular functions. It excels in supporting robust transcription, making it an ideal site for maintaining the expression of an externally introduced gene while mitigating the risk of unintentional integration elsewhere. Our state-of-the-art control donor vectors feature an EGFP gene strategically placed in reverse orientation downstream of the SFFV promoter and upstream of a poly-A tail. This setup ensures a straightforward pathway for achieving enduring and strong EGFP expression. Furthermore, by strategically integrating the Puromycin selection marker with a splice acceptor (SA) site within the intronic region of the AAVS1 safe harbor locus, independent of its own promoter, Puromycin expression becomes closely tied to intron integration. This significantly reduces the risk of unintended off-target integrations when employing Puromycin selection. The pAAVS1-SA-T2A-Puro-SV40pA-EGFP-SFFV control donor vector, when used in conjunction with the pSpCAS9 nuclease & AAVS1 gRNA vector, enables the seamless integration of EGFP into the AAVS1 safe harbor site, making it an effective integration marker.

Vector Information

This AAVS1 donor vector includes essential components to serve as a reliable integration marker in the AAVS1 Safe Harbor site. It incorporates a universal SA-T2A-Puro cassette, facilitating the integration of EGFP driven by an SFFV promoter into the AAVS1 safe harbor site through antibiotic selection. This vector is suitable for generating stable cell lines.

IMPORTANT NOTICE

Store the vial at -20°C immediately upon receipt.

images/Desc_Images/AC9411_1.png
Note: Bacterial culture of AAVS1 vectors should be done in medium containing 100 μg/mL Carbenicillin. For maximal plasmid yield and quality, we recommend Stbl3 competent cells (Invitrogen).
Specifications:
Product Name pAAVS1-SA-T2A-Puro-SV40pA-EGFP-SFFV Control Donor Vector
Shipping Condition

Room Temperature - 2 Day Shipping

Storage and Stability

Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

This plasmid is sequence verified.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.