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Human iPS Cells (Inducible Ngn2, ApoE3)

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Catalog# Unit Unit Price (USD) Actions
iP26NA 5x105 cells/vial $14,900.00 Add to Cart
Highlights:

ALSTEM offers human iPS cell line with inducible expression of Ngn2 by doxycycline (Dox). This cell line is generated from pre-made footprint-free human iPS cells (cat# iPS26, ApoE3 isoform) with the inducible Ngn2 that has been stably integrated into the human AAVS1 “Safe Harbor” site. It provides a convenient means to differentiate human iPS cells to excitatory neurons rapidly and efficiently. The integration at “Safe Harbor” sites ensures safe and reliable expression of Ngn2 when Dox is supplied.

  • Highly responsive doxycycline inducible iPS cell line
  • Ideal for rapid in vitro neuronal differentiation
  • Especially useful for studying ApoE variants and their roles in neurologic diseases
Description:

Neurogenin (Ngn) 2 is a basic helix-loop-helix (bHLH) transcription factor that promotes direct differentiation of pluripotent stem cells to functional excitatory neurons. ALSTEM’s highly responsive doxycycline inducible Ngn2-expressing ApoE3 cell line (cat# iP26NA) is produced from pre-made footprint-free human iPS cells (Cat# iPS26) by integrating an all-in-one inducible Ngn2 cassette into the human AAVS1 “Safe Harbor” site, which supports robust and sustained expression of the inserted gene and allows for rapid neuronal differentiation upon induction.

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Alstem’s all-in-one donor plasmid consists of both inducible Ngn2 and constitutive M2rtTA that have been inserted into one of the AAVS1 alleles, while the other allele remains intact. Upon induction, Ngn2 is expressed and the iPS cells will be directly differentiated into excitatory neurons.
Specifications:
Product Name Human iPS Cells (Inducible Ngn2, ApoE3)
Shipping Condition

Dry Ice - Overnight Shipping
Storage and Stability

Store in the vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control

Human iPS Cells (Inducible Ngn2, ApoE3) were grown in feeder-free conditions with mTeSR1 medium. Each lot of hiPSCs is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and OCT4.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.