KCNK3 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)
| Catalog# | Unit | Unit Price (USD) | Actions |
|---|---|---|---|
| iPS51 | 5x105 cells/vial | $2,835.00 | Add to Cart |
Highlights:
ALSTEM offers human iPS cell line (iPS51) with KCNK3 deficiency. This cell line is generated from pre-made footprint-free human iPS cells (cat# iPS11) by using gene editing method to introduce a deletion in the exon 2 of KCNK3 that resulted in a frame shift mutation. DNA sequencing results confirmed that this isogenic iPSC line has a stable homozygous 142 bp deletion in the exon 2 of KCNK3 gene. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells.
- Good model for studying pulmonary hypertension-related pathologies in hepatic ABC transporters and aldosterone synthesis and secretion pathways
- Ideal for drug discovery when used with KCNK3 wild type iPS cell line (cat# iPS11)
Description:
Human KCNK3 gene (potassium two pore domain channel subfamily K member 3) encodes a pH-sensitive potassium channel in the 4-transmembrane/2-pore domain superfamily. The primary role of KCNK3 channels is to control the resting membrane potential in many cell types, including pulmonary artery smooth muscle cells. Mutations of KCNK3 are associated with Pulmonary Hypertension-4 (PPH4), a rare genetic progressive disease characterized by increased pulmonary artery pressure in the absence of other in the absence of common causes of pulmonary hypertension, such as chronic heart, lung, or thromboembolic disease. KCNK3 is related to several pathways such as Hepatic ABC Transporters and Aldosterone synthesis and secretion.
ALSTEM’s footprint-free human iPS cell line (cat# iPS51) carries KCNK3 KO mutation in both alleles. Sequencing results confirmed that this isogenic iPSC line has a stable homozygous 142 bp deletion in the exon 2 of KCNK3 gene. The morphology of this human iPS cell line is identical to that of human ES cells. The cells also express the pluripotency markers TRA-1-60, SSEA-3 and OCT4. It also demonstrates strong endogenous alkaline phosphatase activity.
Specifications:
| Product Name | KCNK3 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF) |
|---|---|
| Shipping Condition |
Dry ice - Overnight Shipping |
| Storage and Stability |
Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed |
| Quality Control | Human iPS cells were grown in feeder free conditions with mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of TRA-1-60 and OCT4, as well as the activity of alkaline phosphatase. |
| Restricted Use |
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |